Multiple ligation-Assisted recombinase polymerase amplification for highly sensitive and selective colorimetric detection of SARS-CoV-2Article Published on 2023-01-152022-11-15 Journal: Talanta [Category] SARS, 치료기술, 치료제, [키워드] acute respiratory syndrome amplification Analysis Bacterial colorimetric detection Colorimetric sensing conditions coronavirus detection system diagnosing disease Diseases effective Genome isothermal isothermal amplification limit of detection Multiple PK-probe point-of-care polymerase probe Pyrophosphate recombinase polymerase amplification Recombinase polymerase amplification (RPA) RNA robust SARS-CoV-2 SARS-CoV-2. selective sensitivity [DOI] 10.1016/j.talanta.2022.123835 PMC 바로가기
Evaluation of indirect sequence-specific magneto-extraction-aided LAMP for fluorescence and electrochemical SARS-CoV-2 nucleic acid detectionArticle Published on 2023-01-152022-11-15 Journal: Talanta [Category] SARS, 변종, 치료기술, 치료제, [키워드] amplification approach circumvent clinically Disadvantages Electrochemical LAMP Host in silico inhibitor investigated isothermal isothermal amplification Magnetic sequence-specific target enrichment molecular NAAT nucleic acid Nucleic acid detection PCR polymerase probe qRT-PCR Quantitative Quantitative real-time LAMP real-time reverse transcriptase restrict SARS-CoV-2 SARS-CoV-2 nucleic acid SARS-CoV-2 variant SARS-CoV-2. sensitivity sequence serum turn [DOI] 10.1016/j.talanta.2022.123809 PMC 바로가기
Highly sensitive and rapid detection of SARS-CoV-2 via a portable CRISPR-Cas13a-based lateral flow assayArticle Published on 2022-12-012022-11-15 Journal: Journal of medical virology [Category] COVID19(2023년), SARS, 치료기술, [키워드] acute respiratory syndrome addition amplification approach assays clinical sample comparable component Concentration coronavirus disease Coronavirus-2 COVID-19 CRISPR Diagnostic method Effectiveness ENhance examined identify immunochromatographic IMPROVE individual isothermal lateral flow lateral flow assay lateral flow strip limit of detection molecular polymerase chain primer probe Protein provide Quality control reliability Result reverse transcription RNA RT-PCR SARS-CoV-2 screened sensitive detection. sensitivity significantly Spread tested virus detection [DOI] 10.1002/jmv.28096 PMC 바로가기
Cas12a-assisted RTF-EXPAR for accurate, rapid and simple detection of SARS-CoV-2 RNAArticle Published on 2022-11-152022-11-15 Journal: Biosensors & bioelectronics [Category] SARS, 치료기술, [키워드] amplification Analysis approach COVID-19 pandemic CRISPR CRISPR-Cas12a detect Detection limit Detection sensitivity Developing End-point fluorescence equipment identify increase intrinsic isothermal Isothermy molecular point-of-care reported RGB RTF-EXPAR SARS-CoV-2 SARS-CoV-2 infected patient SARS-CoV-2 RNA SARS-CoV-2 RNA detection SARS-CoV-2 RNA. specificity [DOI] 10.1016/j.bios.2022.114683 PMC 바로가기
One-step colorimetric isothermal detection of COVID-19 with AI-assisted automated result analysis: A platform model for future emerging point-of-care RNA/DNA disease diagnosisResearch article Published on 2022-11-012022-10-05 Journal: Talanta [Category] SARS, 변종, 유전자 메커니즘, 치료기술, [키워드] Accuracy Analysis analyzed approach automated Colorimetric RT-LAMP Complete Concordance COVID-19 COVID-19 testing cycle threshold Detection limit diagnosis of COVID-19 Disease diagnosis DNA downstream ENhance exhibited faster feasibility Interpretation isothermal LAMP-DETR limit machine learning majority observation offer pandemic pathogen performed Perspective platform point-of-care RT-LAMP RT-qPCR SARS-CoV-2 sensitivity specificity [DOI] 10.1016/j.talanta.2022.123375 [Article Type] Research article
Development of a Loop-Mediated Isothermal Amplification Method for Rapid and Visual Detection of Monkeypox VirusArticle Published on 2022-10-262022-11-16 Journal: Microbiology Spectrum [Category] 치료기술, [키워드] amplification caused clinical sample clinical samples COVID-19 pandemic detection Detection limit development Diagnosis early stage evaluated hospital Infection isothermal isothermal amplification Loop-mediated isothermal amplification Monkeypox Monkeypox virus orthopoxvirus outbreak pathogen pathogenic virus Pathogens PCR primer set Primers public health threat Rapid rapid detection sensitivity sequence specificity Symptom temperature virus Visual visual detection. [DOI] 10.1128/spectrum.02714-22 PMC 바로가기
Reverse Transcription-Loop-Mediated Isothermal Amplification-CRISPR-Cas13a Technology as a Promising Diagnostic Tool for SARS-CoV-2Article Published on 2022-10-262022-11-16 Journal: Microbiology Spectrum [Category] COVID19(2023년), SARS, 치료기술, [키워드] acute respiratory syndrome caused change clinical samples coronavirus coronavirus disease COVID-19 CRISPR-Ca CRISPR-Cas CRISPR-Cas13 cycle threshold deaths develop Diagnosis diagnosis. diagnostic Diagnostic method early diagnosis effort exhibit highlighting isothermal isothermal amplification maintain Molecular diagnosis nasopharyngeal sample Negative predictive value New coronavirus NPV pandemic performed persist Positive predictive value PPV Prevalence protocol reaction required researcher RNA extraction RNA molecules RT-LAMP SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 virus sensitivity Sensitivity and specificity single nucleotide single-stranded specificity technology viral loads [DOI] 10.1128/spectrum.02398-22 PMC 바로가기
PrimedSherlock: a tool for rapid design of highly specific CRISPR-Cas12 crRNAsArticle Published on 2022-10-142022-11-15 Journal: BMC Bioinformatics [Category] COVID19(2023년), SARS, 치료기술, 치료제, [키워드] assays automated benefit bioinformatics Cas12 nuclease assay Cas12a Combination conserved CRISPR CRISPR-Ca CRISPR-Cas12 Critical demonstrated Dengue diagnostic diagnostics Efficiency facilitated FDA approval field Field forward diagnostics flavivirus genomes genomic HIV In-vitro Intensive isothermal isothermal amplification manual curation Nile nucleases nucleic acid offer parameter Pathogens platform polymorphism PrimedRPA PrimedSherlock primer primer pair Primers qRT-PCR regions RNA robust SARS-CoV-2 Serological assay serotype SHERLOCK significantly specificity strain Strains target targets Vector-borne disease West West Nile wildtype Zika Zika. [DOI] 10.1186/s12859-022-04968-5 PMC 바로가기
Real-time monitoring of isothermal nucleic acid amplification on a smartphone by using a portable electrochemical device for home-testing of SARS-CoV-2Article Published on 2022-10-092022-11-15 Journal: Analytica chimica acta [Category] COVID19(2023년), SARS, 치료기술, [키워드] acute infectious disease approach automatically Bluetooth caused comparable criteria demonstrated Electrochemical detection exhibited Infectious disease isothermal Isothermal nucleic acid amplification linear nucleic acid nucleic acid amplification pandemic of COVID-19 pH value pH-sensitive potentiometric sensor. Protein Real-time monitoring resource SARS-CoV-2 SARS-CoV-2 detection SARS-CoV-2 nucleocapsid sensitivity Smartphone utility [DOI] 10.1016/j.aca.2022.340343 PMC 바로가기
A quantitative RT-qLAMP for the detection of SARS-CoV-2 and human gene in clinical applicationArticle Published on 2022-10-012022-11-16 Journal: Microbial Biotechnology [Category] COVID19(2023년), SARS, 변종, 치료기술, [키워드] applied BBIBP-CorV Beta clinical sample clinical samples Clinical treatment cycle threshold Delta demonstrated detect evaluate evaluated fluorescent full-length help human gene inactivated isothermal isothermal amplification linear linear relationship management omicron pandemic Patient physician positive qPCR Quantitative required RNA RT-qPCR SARS-CoV-2 swab samples threshold Transcription variants of SARS-CoV-2 Viral load viral loads viral particle was used [DOI] 10.1111/1751-7915.14112 PMC 바로가기